LC/MS characterization of meningococcal depolymerized polysaccharide group C reducing endgroup and internal repeating unit.

Chaperonin (MSP63) complexes induce bactericidal and opsonophagocytic cross-reactive antibodies

Methods to identify intact multi-protein complexes can help to achieve more effective formulations but their application to OMVs can be difficult due to the hydrophobicity of membrane proteins. Our previous studies of the meningococcal porin complexes using blue native polyacrylamide gel electrophoresis (BNE) (1) allowed the detection of a 700 kDa major complex. Analysis by LC/MS-MS revealed th...

An oligosaccharide-tetanus toxoid conjugate vaccine against type III group B Streptococcus.

We have developed an oligosaccharide-tetanus toxoid conjugate vaccine against type III group B Streptococcus. Purified group B streptococcal type III capsular polysaccharide was depolymerized by enzymatic digestion using endo-beta-galactosidase produced by Citrobacter freundii. Following enzymatic digestion, oligosaccharides were fractionated by gel filtration chromatography on Sephadex G-75. A...

Synthesis of Neisseria meningitidis serogroup W135 capsular oligosaccharides for immunogenicity comparison and vaccine development.

Neisseria meningitidis (meningococcus) is a Gram-negative human pathogen that causes meningococcal diseases, such as meningococcal meningitis and meningococcal septicemia. Based on the surface capsular oligosaccharides of the organism, 13 serogroups of N. meningitidis have been identified, among which A, B, C, Y, and W135 are the major pathogenic strains. Serogroup A is the pathogen most often ...

Structural determination and immunochemical characterization of the type V group B Streptococcus capsular polysaccharide.

The type V capsular polysaccharide of group B Streptococcus has been isolated and purified, and its repeating unit structure determined. The native type V polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in a molar ratio of 3:2:1:1. Methylation analysis and 1H NMR and 13C NMR analysis of the native type V polysaccharide and of its specifically degra...

Immunogenicity in Mice Conjugates and Evaluation of Their A Meningococcal Capsular Polysaccharide Preparation and Characterization of Group